C/EBPα-ER favors monocytic development as assessed by RNA analysis. Total cellular RNAs were isolated from marrow cells transduced with pBabePuro or pBabePuro-C/EBPαER followed by lineage-depleted, and culture with or without 1 μM E₂ for 2 days. After first-strand cDNA synthesis, quantitative PCR was carried out in 3 separate runs for the indicated mRNAs and GAPDH. Fold increase was calculated as the ratio of normalized signal with E₂/without E₂. Mean values and SE are shown.