Ang2 inhibited Ang1-induced HGF expression in endothelial cells. (A) HUVECs were infected with different viral vectors expressing genes of interest for 48 hours. Total RNAs were extracted from the cells. HGF mRNA was analyzed by Northern blotting and was probed with HGF cDNA. 18S rRNA, visualized with ethidium bromide and UV, was used as a loading control. (B) Cell-conditioned media were collected, concentrated, and analyzed for protein expression by Western blotting. Filters were immunoblotted with anti-Ang1– and anti-Ang2–specific antibodies, respectively. Representative images are shown. Experiments were performed at least 3 times.