Plasma VWF multimer pattern obtained by high-resolution SDS-agarose gel electrophoresis for affected and unaffected family members. (A) Plasma VWF multimer distribution of members of families A and B. Affected family members harbor a S2179F VWF mutation and are denoted in bold type above each lane. Multimers in plasma from Patient AIII-1 were obtained before (T = 0) and at various time points after DDAVP administration. (B) Plasma VWF multimer distribution of members of family C. Affected family members harbor a W1144G VWF mutation and are denoted in bold type above each lane. At steady-state, VWF multimer satellite bands are nearly absent from the plasma of affected family members.