SP analysis of CD34^-KSL cells in mouse BM. Flow cytometric analysis of CD34^-KSL cells stained with Hoechst 33342 is shown. (A) Lin⁺ cell-depleted mouse BM cells are displayed for c-Kit and Lin expression with the sorting gate for Lin^- cells. (B) Lin^- cells are displayed for Sca-1 and c-Kit expression with the sorting gate for Sca-1⁺c-Kit⁺ cells. TR indicates Texas red. (C) The histogram shows the CD34 expression of KSL cells with the sorting gate for CD34^- cells. (D) Lin^- cells are displayed for Hoechst red and blue. (E) KSL cells are displayed for Hoechst red and blue. (F) CD34^-KSL cells are displayed for Hoechst red and blue. The sorting gates for the SP and MP phenotypes among CD34^-KSL cells are presented.