SP analysis of KSL cells derived from SP- or MP-CD34^-KSL cells. (A) BM cells from mice reconstituted with 30 SP-CD34^-KSL cells were analyzed 4 months after transplantation. (B) BM cells from mice reconstituted with 30 MP-CD34^-KSL cells are analyzed simultaneously. Test donor cells are identified as Ly5.2 cells. Sequential gating is shown from the left to the right panels, with Ly5.2-KSL cells (staining with both Hoechst red and Hoechst blue) in the panel at far right.