Figure 1.
![Figure 1. Effect of high extracellular [K+] on collagen-plus-thrombin–induced platelet procoagulant response. Platelets at 107 mL–1 were activated with collagen (10 μg/mL) plus thrombin (4 nM) for 10 minutes in HEPES, HEPES/choline, or HEPES/KCl, and the procoagulant response was measured as the binding of FITC-conjugated annexin A5. Data are expressed as a percentage of the response obtained in HEPES. We emphasize that the 100% value in HEPES corresponds to approximately 20% to 25% of the total cell population that becomes positive for annexin binding, depending on the donor. Data are mean values ± 1SD(n = 4).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/108/7/10.1182_blood-2006-01-009613/4/zh80190601770001.jpeg?Expires=1735536909&Signature=HIbcdEXAh1zAtrTvC4YdgXY8JExI41akCXfc2opA~S4n39xUubE~ZgNhSugzXn-t4Wopxt6niaJUXjodZ~aHMz~A2TdLUbJaV2Rbqg2inbwip4CnoqmS8B9nW-iSPkH7oFM5faWVRBZM3xJv3ljG~tTz~Y20u9iQXZTW5LzyAus5Hfrvv8E22t8W5HjVg5dqfs7rj-PQ8fCg499IavQLWlYfZfig8Yp5vUdBA-5qC8LTIQ59Rdh7YJoCsGer~dHl12bSuFg31lZ9Xj7wh0wn8lNoB-T3pWvkvtXq1G9y~eahrgvhSQZD-79~~hpAoReUvibhUtZ8iF2O9X1K9YCq4Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of high extracellular [K+] on collagen-plus-thrombin–induced platelet procoagulant response. Platelets at 107 mL–1 were activated with collagen (10 μg/mL) plus thrombin (4 nM) for 10 minutes in HEPES, HEPES/choline, or HEPES/KCl, and the procoagulant response was measured as the binding of FITC-conjugated annexin A5. Data are expressed as a percentage of the response obtained in HEPES. We emphasize that the 100% value in HEPES corresponds to approximately 20% to 25% of the total cell population that becomes positive for annexin binding, depending on the donor. Data are mean values ± 1SD(n = 4).
