Propagation of BM-derived GFP-positive cells engrafted in the graft GALT. Lethally irradiated WT rats were adoptively transferred with cells isolated from GALT of WT intestine grafts simultaneously transplanted with GFP-positive BM. Flow cytometric analysis of peripheral blood was performed 100 days after irradiation and transfer. After adoptive transfer of cells isolated from MLNs and PPs of naive GFP rats, a GFP-positive population was not identified. On the other hand, multilineage GFP-positive cells including T, B, and monocytes/macrophages (about 6%) were detected after infusion of cells from WT intestine grafts transplanted with GFP-positive BM at day 120. Pictures are representative of 3 different animals of each group.