Macrophages from P1 had abnormal podosome clustering and increased levels of F-actin. (A) Normal macrophages were polarized with podosomes evenly distributed at the leading edge. (B-D) Macrophages from P1 exhibited a loss of polarization and uneven podosome distribution; typically podosomes were found in multiple clusters or ring structures. (E and F) Staining with antivinculin antibody revealed a ring of vinculin around an actin core for both normal and patient cells, respectively. Insets show a magnified view of an individual podosome. (G) Quantification of podosome clusters per cell. (H) Quantification of F-actin in normal and patient cells.