PKH26- and CFSE-tagged donor cells migrate to the peritonea of thioglycollate-elicited recipients. Total bone marrow cells from a Siat1ΔP1 mouse were separately labeled with either PKH26-red or CFSE. The tagged donor cells were recombined, and pooled cells were injected by tail vein into a recipient mouse, also Siat1ΔP1. Concomitant with the transfer of the tagged donor cells, peritonitis was elicited by intraperitoneal injection of 1 mL of 4% (wt/vol) thioglycollate. Peritoneal cells were recovered 6 hours later, and the presence of tagged donor cells was profiled by flow cytometry. (A) Recipient animal that did not receive tagged donor cells. (B) Recipient animal receiving 107 pooled donor cells. Gates (boxed regions, B) were used to determine the PKH/CFSE ratios of tagged cells, as summarized in Table 2. Typically, up to 0.4% of the labeled donor cells are recovered in the peritonea of the thioglycollate-elicited recipients.
