Figure 1.
Bivalent Ab1 remarkably inhibits proliferation of HRMECs. (A) HRMECs were plated in a 12-well plate at the density of 3 × 104 cells/4.0 cm2 (day 0). The medium was replaced every 2 days subsequently (arrows), supplemented with 67 nM (10 μg/mL) Ab1, Ab1.Fab, or mouse IgG1 (isotype-matched control antibody), and the cell number (means ± SEM, n = 6) was evaluated as described in “Materials and methods.” Bivalent Ab1, but not monovalent Ab1.Fab, markedly inhibited proliferation of HRMECs. (B) Cell numbers at day 6 cultured in the growth medium supplemented with indicated concentrations of agents were determined. Means ± SEM of quadruplicate samples are shown. Cell numbers (○) cultured in serum-free medium are also displayed.
