PTCRA expression in APL compared with non-APL AML and T-ALL. Quantification by RQ-PCR of the expression of PTCRA (A), RAG1 (B), and TEA-Cα (C) relative to ABL in T-ALLs, in M3v and classic M3, and in M1/M2 and in M4/M5. Transcript levels are compared with those from normal bone marrow (BM) and peripheral blood (PB) amplified from pure cDNA or after 10% dilution into U937 cDNA (ND indicates not done for RAG1). Results are presented as a box plot graph using a logarithmic scale. The boundary of the box closest to zero indicates the 25th percentile, a line within the box marks the median, and the boundary of the box farthest from zero indicates the 75th percentile. Narrow horizontal bars above and below the box indicate the 90th and 10th percentiles. Outliers are indicated as dots. The number of cases quantified in each category is indicated below the category. Mann-Whitney statistical results comparing each category with M3v's are indicated by P value.