The persistence of donor-derived DCs in draining lymph nodes is impaired in the presence of CD8 T cells. Mice were injected in the hind footpads with semi-allogeneic CB6F1 DCs. (A) The presence of injected DCs was monitored by flow cytometry analysis of the draining lymph node cells (pooled from 3 mice per group) after 72 hours. The percentage of I-E^d–positive DCs, gated on CD11c⁺, MHC II^high after propidium-iodide–positive cell exclusion, is shown (mean ± SEM of 4 different experiments). (B) Kinetics of DC recruitment into the draining lymph nodes of WT, CD8^–/–, or CD8-depleted mice at 24, 48, and 72 hours after injection. (C) DCs were pretreated or not with pertussis toxin and injected into WT or CD8^–/– mice. At 48 hours after injection, draining lymph nodes were analyzed for the presence of injected I-E^d–positive CD11c⁺, MHC^High DCs. Data are from 1 representative experiment of 3 performed.