Host CD8 T cells eliminate donor-derived DCs through a perforin-dependent mechanism. WT, IFN-γ^–/–, or Pfp^–/– B6 mice, treated or not with anti-CD8 mAb, were injected with equivalent numbers of CFSE^low-labeled syngeneic B6 DCs and CFSE^high-labeled semi-allogeneic CB6F1 DCs. Draining lymph nodes were harvested at 24 or 72 hours after injection and analyzed for the presence of CFSE-labeled cells. (A) Cells were analyzed at 72 hours and gated on CD11c^pos, MHC-II^high, and CFSE^pos cells. Numbers indicate the percentage of CFSE^high CB6F1 cells among total CFSE^pos cells for an individual mouse. (B) Results are expressed as mean plus or minus SEM of 3 mice per group and are from 1 representative experiment of 2 performed.