Generation of Vγ3⁺T cells in the thymus and skin of CCR7/CCR9-deficient mice. (A) E15.5 fetal thymocytes (top panels) and 7-day-old newborn thymocytes (P7, bottom panels) isolated from indicated mice were stained for CD45 and either one of TCR-Cδ (for total γδ), Vγ3, or Vγ2. Cell number of indicated population was calculated from total viable thymocyte number and the frequency of that population. Symbols indicate the number of indicated thymocyte populations in individual mice, and bars indicate their means. (B) Epidermal cells isolated from abdomen skin of 7-day-old (P7) newborn mice or ear skin of 7- to 9-week-old adult mice were stained for TCR-Cδ or Vγ3. Symbols indicate the frequency of indicated cell populations within the total viable epidermal cells, and bars indicate means plus or minus standard errors. (C) Representative flow cytometry profiles of indicated thymocytes and epidermal cells are shown. Numbers in the dot plots indicate the frequency of cells within the quadrant. (D) Representative immunofluorescence images of 7-day-old epidermal sheets of Vγ3⁺ T cells or I-A⁺ Langerhans cells were visualized (FITC signals in green) in epidermal sheets (background signals in blue) from indicated mice. Images were obtained using a 63 ×/1.40-0.60 oil objective. ^**P < .005; ^***P < .001; NS, not significant.