LBH589 potently induces histone and α-tubulin hyperacetylation as well as apoptotic signaling. MM.1S cells were incubated with up to 100 nM LBH589 for 24 hours, and whole-cell extracts were analyzed by Western blot. (A) There was a dose-dependent increase in both histone acetylation (top panel) and α-tubulin acetylation (bottom panel). (B) Cleavage of caspase-8, -9, -3 and PARP was induced in a dose-dependent manner. In addition, p21 was up-regulated and c-myc was down-regulated. FL indicates full length; CL, cleavage fragment.