Decreased expression of TAL1 alters human hematopoietic recovery after transplantation into NOD-SCID mice. Transduced CD34⁺ cells were injected into NOD-SCID mice for SRC analysis. FACS analysis of mice bone marrow was performed 6 weeks and 12 weeks after transplantation. (A) Engraftment of human CD45⁺/GFP⁺ and human CD45⁺/GFP^– cells in 2 representative mice 12 weeks after injection of shRNActrl or shRNATAL1 transduced CD34⁺ cells. (B) Engraftment levels of GFP⁺ cells for mice that underwent transplantation either intravenously (•) or intrabone (○); the horizontal bars indicate median % of CD45⁺/GFP⁺ cells measured for injected mice of each condition. (C) When the level of engraftment was sufficient, the relative proportion of myeloid CD14⁺/CD15⁺ and B-lymphoid CD19⁺ cells issued from ST-SRCs and LT-SRCs was determined. Lower and upper SE bars are related to CD14⁺/CD15⁺ and CD19⁺ cells, respectively.