Effects of S 36578-2 on HDMECs and HPMECs spread on vitronectin. The compound S 36578-2 was added to cells 3 hours after seeding cells in experimental medium. Floating and attached cells were collected 24 hours after treatment. Apoptosis was assessed by flow cytometry after annexin V and propidium iodide staining in HDMECs (A) and HPMECs (B). Results of 2 experiments (1 per donor) are shown. (C) Phase contrast micrographs were taken 24 hours after treatment. Scale bar represents 100 μm. Results of 2 experiments (1 per donor) are shown.