Figure 2.
MtFt expression affects heme metabolism of tumor xenografts. Total heme content in the tumors (A) and in the livers (B) of tumor-bearing mice were measured by fluorescent spectrophotometry.37 Tumor and liver lysates were incubated with 2 M oxalic acid for 30 minutes in a boiling water bath, following which the fluorescence of heme-derived protoporphyrin IX was measured using 662 nm emission and 400 nm excitation (*P < .05). (C) Effect of MtFt expression on HO-1 protein expression. Tumor lysates identical to those in panel A were used to measure HO-1 protein levels by Western blotting.
