Effect of G-CSF treatment on neutrophil chemotaxis function recovery after BMT. This chart shows the mean number of neutrophils recruited to the peritoneal cavity following the introduction of NaOI in SV129/black mice with and without G-CSF treatment after BMT. Neutrophils were counted by a Coulter Z2 particle counter (Beckman Coulter Canada). The mean number of neutrophils recruited in untreated recipient control mice is represented by the dotted line (8.00 × 105 cells/mL). G-CSF treatment resulted in the increased recruitment of neutrophils to the peritoneum to reach normal levels (8.00 × 105 cells/mL) faster on days following BMT than when no G-CSF treatment was administered. The t tests revealed that G-CSF treatment resulted in a 3-fold significant increase in neutrophil chemotactic recruitment for day 3 (P < .005) and day 6 (P < .005) and a 2-fold increase for day 7 (P < .05) after BMT. Data are shown as mean ± SEM (3-8 mice per day after BMT).
