HPCs from aged mice are reduced in their ability to adhere to stroma. (A) BM cells from mobilized and nonmobilized aged (21-27 months) and young (2-3 months) mice were subjected to CAFC adhesion assay. Experimental setup. (B) Percentage of adherent HPCs after 2 or 4 hours determined as CAFC day 7 cells using the CAFC adhesion assay. n = at least 3 for each age group. Values shown are mean ± 1 SEM. (C). Ratio of the MFI of CXCR4, CD49d, CD49e on HPCs and HSCs from aged (21-22 months) and young (2-3 months) mice. No change in the MFI of expression between cells from young and aged animals resulted in ratio of 1, indicated by the dashed line. n = 3 mice each aged and young for CXCR4, and n = 4 mice each aged and young for CD49d and CD49e. *P < .05.