Platelet glycoprotein analysis by flow cytometry and WB. (A) PRP from patients 1 and 2 or a control donor were incubated with a battery of MoAbs recognizing α_IIbβ₃ (AP2), α₂β₁ (Gi9), GPIbα (AP1, Alma12, Bx1, WM23), GPIX (FMC25), or the GPIb-IX complex (SZ1). Antibody binding was assessed by flow cytometry. (B) Lysates of platelets from patients 1 (P1) and 2 (P2) and a control donor were analyzed by SDS-PAGE and immunoblotted with MoAbs to β3 and GPIbα. (C) Platelets from patients 1 and 2 or a control donor were sedimented and resuspended in washing buffer prior to incubation with a polyclonal antibody to VWF, a MoAb to P-selectin (VH10), and anti-LIBS and anti-RIBS MoAbs, respectively. Flow cytometric histograms are shown.