Generation of SALL4B transgenic mice. CMV/SALL4B transgenic construct and PCR analysis of transgenic line 507. (A) Schematic diagram of transgenic construct. The approximately 1.8-kb cDNA of SALL4B was subcloned into a pCEP4 vector, and the CMV/SALL4 construct was excited by digestion with SalI. (B) Tissue distribution of SALL4B in transgenic mice. The location of primers used for RT-PCR amplification is indicated by arrows in panel A. A primer specific for human SALL4B at the C-terminus was used as a 5′ primer, in combination with SV40-noncoding sequence-specific primers for RT-PCR of various tissues.