Figure 3.
Memory B-cell response to immunization. (A) MenC polysaccharide vaccine. (B) MenC polysaccharide/CRM197 conjugate vaccine. Subjects were immunized on day 0, and PBMCs were separated on various days after immunization and cultured with SAC/IL2 for 5.5 days before MenC-specific ELISpot assays. Numbers of subjects whose PBMCS were obtained for ELISpot for each day after immunization are given in the table below the chart. Different subjects were studied at each time point. B-cell numbers are expressed as MenC-specific ASCs per 2 × 105 cultured lymphocytes. Horizontal bars represent the median number of MenC-specific ASCs at each time point. Blank cells are as in Figure 1.
