Analysis of NRASD12 proviral integration in AML and CMML tissues. (A) For analysis of NRASD12 proviral integration in diseased mice, genomic DNA from the livers of NRASD12 mice that succumbed to AML-like disease (lanes 2-5) and CMML-like disease (lanes 6-9) was digested with BglII and probed with a 0.7-kb ³²P-labeled GFP probe. Lane 1 is a control to establish levels of a single copy of the provirus. The blot was then stripped and reprobed with a 1.4-kb ³²P-labeled irf4 probe as a loading control (bottom). (B) Genomic DNA was digested with XbaI, which cuts within the LTRs, to show intactness of the provirus. The top panel shows the MSCV-BCR/ABL-IRES-GFP single copy provirus control, which is larger in size than the RAS provirus. The blot was stripped and reprobed with an irf4 probe as a loading control (bottom). The relative ratios of proviral DNA to the irf4 loading control as compared with that of the cell line control are shown.