Impaired IL-12p70 production in diffDCs is not due to high IL-10 production. (A) imDCs and diffDCs were pretreated with 10 μg/mL anti–IL-10 antibody or isotype antibody for 30 minutes, treated with 500 ng/mL LPS for 24 hours, and then the level of IL-12p70 in supernatants was determined by ELISA. Untreated DCs were used as a control. *P < .05. (B) imDCs and diffDCs from wild-type or IL-10^–/– mice (IL-10^–/– imDC and IL-10^–/– diffDC) were stimulated with various concentrations of LPS (1 ng/mL-500 ng/mL) for 24 hours. Supernatant was measured using an ELISA kit for IL-12p70. Untreated DCs were used as a control. *P < .05.