ERK1/2, JNK1/2 and p38 MAPK pathways are differentially activated in imDCs and diffDCs. imDCs and diffDCs (1 × 10⁶) were treated with 500 ng/mL LPS for 0 to 60 minutes, and were then lysed. The phosphorylation of JNK1/2 (p-JNK1/2), p38 (p-p38), and ERK1/2 (p-ERK1/2) was detected by Western blotting using specific antibodies. Total JNK1/2, p38, and ERK1/2 in each sample were used as the equal loading control. The same blot was used for each different antibody after stripping of the previous antibody. Similar results were obtained in 3 indepedent experiments.