Dectin-1–mediated induction of TNF-α by BMMϕs infected with M smegmatis requires TLR2. BMMϕs from WT or TLR2^–/– mice were infected with M smegmatis or M avium 724 in the presence of anti–dectin-1 mAb 2A11. After a 4-hour infection, BMMϕs were washed, fresh medium was added to the cells, and infection was continued for a total of 24 hours. Culture supernates were analyzed by ELISA for TNF-α. *Significant to M smegmatis plus PBS (P < .01). Values are expressed as means + SD. Data are representative of 3 separate experiments. Smeg indicates M smegmatis; Avium, M avium; and RC, noninfected BMMϕs.