Induced L-selectin shedding by neutrophils from ADAM17ΔZn/ΔZn- and ADAM17+/+-chimeric mice. (A) Peripheral-blood leukocytes from the chimeric mice were treated with or without PMA for 20 minutes, as indicated, and then double-stained for surface expression of L-selectin (left row) or Mac-1 (right row) and neutrophil marker Ly-6G. (B) Peripheral-blood (pb) and peritoneal-cavity (pc) neutrophils from the chimeric mice following 2 hours of thioglycollate-induced peritonitis were double-stained for surface expression of L-selectin and neutrophil marker Ly-6G. Relative staining levels were determined by flow cytometry. For all histogram plots, the dashed line indicates staining by an isotype-matched negative control mAb. The y-axis indicates cell number and the x-axis indicates log-10 fluorescence. Results are representative of 3 or more independent experiments.
