T-cell subset analyses in hu-PBL-NOD/SCID mice after NP-IDV-BMM treatment. (A) Flow cytometric histograms of splenocytes from mice 14 days following NP-IDV-BMM treatments were used to determine the numbers of mouse CD45 cells and human reconstituted CD4⁺ and CD8⁺ T cells. (B) The level of human lymphocyte reconstitution in mouse spleens was determined compared with mouse CD45⁺ cells. (C-D) Frequencies of (C) CD4⁺ and (D) CD8⁺ T cells were assessed as percentages of total human T cells. (E) Ratios of CD4⁺/CD8⁺ T cells increased significantly in NP-IDV-BMM-treated group compared with control animals. Data represent mean ± SEM for 4-6 mice/group. ^*P < .05 and ^**P < .01 compared with untreated controls.