Figure 4.
Figure 4. Representative laser-scanning confocal micrographs of carotid arteries stained with DHE. Sections of carotid artery were imaged with a laser scanning confocal microscope using a 10×/0.3 NA objective lens (BioRad, Hemel Hampstead, United Kingdom); images were acquired with BioRad Laser Sharp 2000 software. (A) Cbs+/+ mice fed the control diet. (B) Cbs+/– mice fed the control diet. (C) Cbs+/+ mice fed the HM/LF diet. (D) Cbs+/– mice fed the HM/LF diet. (E) Cbs+/– mice fed the HM/LF diet; sections preincubated with PEG-SOD. Scale bar represents 50 μM. (F) Relative DHE fluorescence (n = 6 in each group). Values are mean ± SEM.

Representative laser-scanning confocal micrographs of carotid arteries stained with DHE. Sections of carotid artery were imaged with a laser scanning confocal microscope using a 10×/0.3 NA objective lens (BioRad, Hemel Hampstead, United Kingdom); images were acquired with BioRad Laser Sharp 2000 software. (A) Cbs+/+ mice fed the control diet. (B) Cbs+/– mice fed the control diet. (C) Cbs+/+ mice fed the HM/LF diet. (D) Cbs+/– mice fed the HM/LF diet. (E) Cbs+/– mice fed the HM/LF diet; sections preincubated with PEG-SOD. Scale bar represents 50 μM. (F) Relative DHE fluorescence (n = 6 in each group). Values are mean ± SEM.

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