CD44^hiCD8⁺T lymphocytes preferentially expand in DNRII Tg mice. (A) Lymphocytes from 6-week-old DNRII Tg mice or nontransgenic littermates (C57BL/6) were analyzed for CD44^hi cell-surface expression (MFI, greater than 100) on CD4⁺ or CD8⁺ T-cell subsets. Data are represented as the average percentage of CD44^hi cells in each T-cell subset, with error bars representing the SD in each group. (B) Lymphocytes from the same 6-week-old DNRII Tg mice or nontransgenic littermates (C57BL/6) were analyzed for the percentages of CD4⁺ or CD8⁺ T-cell subsets. Data are represented as the average percentage of each T-cell subset among the total T-cell population, with error bars representing the SD in each group. (C) Lymphocytes from 6-week-old DNRII Tg mice were gated into CD8⁺ T-cell subsets (left) or CD4⁺ T-cell subsets (right). Data in the top 2 panels represent the percentage of each T-cell subset with respect to total T cells in the sample. The bottom 6 panels represent flow cytometric analysis of surface receptor expression in each of the 2 gated T-cell subsets (filled histograms). Data represent the percentages of indicated surface receptors within each gated T-cell subset and are typical of these strains of mice at 6 weeks of age. Open histograms indicate nonspecific control staining. (A-B) Average of 5 or more mice per group; experiments repeated 5 times.