Flow cytometric analysis of CD8⁺ T cells from CD8β KO × DNRII Tg mice. Lymphocytes from a representative CD8β KO × DNRII Tg mouse (right panel), a CD8β KO mouse (center panel), and a DNRII Tg mouse (left panel) were analyzed for CD8α and CD8β chains (top panels) or CD44 surface expression on the CD8α-gated lymphocytes (bottom panels). Percentages of gated CD8α⁺ cells (top panels) are denoted by the numbers adjacent to the indicated oval gates and are represented as percentages of total lymphocyte numbers. Percentages of CD44^hi cells in the CD8α population (bottom panels) are indicated to the left of the CD44^hi gates. Data represent typical (n > 10) flow cytometric analysis of CD8α⁺ cells from these strains of mice. All mice studied were older than 16 weeks of age.