Clonal analysis of primary transplanted SRCs. (A) Study design for clonal analysis of primary grafts. 34 indicates CD34⁺ stem/progenitor cells; M, CD33⁺ myeloid lineage cells; B, CD19⁺ B-lymphoid lineage cells; T, CD3⁺ (spleen) or CD4/CD8 double-positive (thymus) T-lymphoid lineage cells. (B) Representative LAM-PCR profiles of SRCs. Each band represents a different insertion locus in the assayed material. W indicates unseparated whole BM MNCs; M, size marker. (C) DP-derived T-lymphoid insertion sites were traced by PCR. The clones detected in all lymphomyeloid lineage cells were designated as multipotent type (MTB). TB indicates clones restricted in T-lymphoid and B-lymphoid cells; T, clones detected in T-lymphoid cells; W, unseparated whole BM MNCs; M, size marker; P, TA-cloned LAM-PCR product was used as a positive control; N, DW. (D) Relative frequencies of each clone type detected in primary SRCs. Data represent mean ± SD of 3 independent experiments. *P < .01 relative to other type of clones. (E) The proportion of clones detected in the CD34⁺ cell population. A total of 27 clones in 3 independent experiments were analyzed. Gray bars represent the clones detected in CD34⁺ cells. Black bars represent the clones not detected in CD34⁺ cells. *P < .01 relative to other type of clones.