Chemotactic activity and CXCR1 receptor expression on neutrophils. (A) Chemotactic response of septic neutrophils (▦, n = 20) or control cells (□, n = 15) toward FMLP (10–7 M), LTB4 (10–8 M), or IL-8 (10–9 M), or medium alone (C indicates controls) in a microchemotaxis chamber. Data are means ± SD. *P < .01 compared with respective control. (B) IL-8 receptor gene expression. Expression of CXCR1 mRNA in neutrophils from controls (□, n = 7) and patients with sepsis (▦, n = 16) evaluated by RT-PCR as described in “Patients, materials, and methods.” Results are shown as units of β-actin mRNA. Insert shows 2 representative experiments showing CXCR1 and β-actin mRNA expression in neutrophils from controls (C1, C2) and patients with sepsis (P1, P2). (C) Flow cytometric analysis of CXCR1 receptor expression on neutrophils from controls (□, n = 8) and patients with sepsis (▦, n = 13). Results are shown as median fluorescence intensity (MFI). Error bars indicate SD.
