Effects of LTB4 and IL-8 on actin-assembling and protein tyrosine phosphorylation of neutrophils treated in vitro with cytokines and LPS. Neutrophils from healthy individuals were incubated with medium alone (i-iii) or with cytokines + LPS (iv-vi) and further stimulated with IL-8 (10–9 M; ii, v) or LTB4 (10–8 M; iii, vi), as described in “Patients, materials, and methods.” F-actin, stained with TRITC-phalloidin (A, C; left panels), and tyrosine-phosphorylated proteins, immunolabeled with FITC-conjugated antiphosphotyrosine Ab (B, D; right panels), were analyzed by fluorescence microscopy (magnification: × 1000) and quantified as described. Data are means ± SD from 10 independent experiments. *P < .05 compared with control neutrophils; #P < .05 compared with cells incubated with medium alone (ANOVA followed by Bonferroni).
