Increase of GRK2 and GRK5 expression in septic neutrophils. (A) Neutrophils from controls (i, iii) or patients with sepsis (ii, iv) were immunostained for GRK2 (i-ii) and GRK5 (iii-iv). The panels show a representative experiment out of at least 7 independent experiments. (B) Immunoblotting for GRK2 (top panel) and GRK5 (bottom panel) in neutrophils from patients with sepsis (▦) or healthy volunteers (□). The blots, obtained from 2 representative experiments, show GRK2 and GRK5 expression in neutrophils from controls (C1, C2) and patients with sepsis (P1, P2). (C) Immunoblotting for GRK2 (top) and GRK5 (bottom) in neutrophils from healthy volunteers incubated with cytokines and LPS, plus IL-8 (10–9 M) or LTB4 (10–8 M), as described in “Patients, materials, and methods.” For immunoblotting, the densitometry of each band, expressing the content of GRK2 and GRK5, was analyzed and expressed in arbitrary units (AU). Data show means ± SD of at least 6 experiments. *P < .05 compared with nontreated cells (ANOVA followed by Bonferroni).
