Long-term engraftment of transduced cells in peripheral blood and lymphoid organs. (A) Quantitative PCR for donor cells (Y chromosome in sex-mismatched transplantation) and transduced cells (LV DNA) in peripheral blood–nucleated cells. Results obtained in different mice assessed at the indicated time are given as percentage of donor cells and vector copy per genome of donor cell, respectively. Horizontal bars represent the average values. Only 2 mice (GT) and 3 mice (BMT) were studied at 28 weeks because the remaining were killed 6 months after treatment for tissue analysis. (B) Donor cell engraftment and vector copy number in primary and secondary lymphoid organs studied at 6 months after GT and BMT. Results are given as the mean ± SD of the percentage of donor cells and vector copy per genome of donor cell in different mice, respectively. Analysis was performed in thymocytes (Thy), T cells (T), and B cells (B) from spleen and lymph nodes, as well as in B cells, myeloid cells (M), and erythroid cells (E) from the BM.