In vitro T-cell functions. (A) Proliferation of splenic T cells in response to TCR stimulation 6 months after treatment. Values are expressed as mean ± SD of stimulation index (calculated dividing cpm numbers of stimulated and unstimulated conditions), obtained from ³H-thymidine incorporation. (B) Cytokine production of splenic T cells. Total splenocytes from ADA^–/– mice at 6 months after GT (ADA^–/– GT; n = 3) or after BMT (^–/– BMT; n = 5) were stimulated with anti-CD3 and anti-CD28 mAbs for 48 hours. Cytokines were measured in culture supernatants by cytometric bead array in triplicate. The values shown are the means and SD of the average of the measurements in the individual mice. As controls, age-matched untreated ADA^+/+ mice (n = 7) and 18-day-old ADA^–/– mice (n = 4) are presented. There was no significant difference among ADA^–/– treated and ADA^+/+ mice.