Chromatin immunoprecipitation and quantitative PCR of the integrated γ-globin gene promoter. GM979 cells were treated with active SCFADs (RB7 or AB) and an inactive SCFAD, RB25. Antibodies against HDAC3 (A) or RNA polymerase II (B) were used for chromatin IP. The results are following a 24-hour exposure to the drugs: control, RB7 (200 μM), arginine butyrate (2500 μM), and RB25 (200 μM). Values are an average of 3 independent experiments and are reported relative to the control. Error bars indicate the standard errors of the mean.