Analysis of antigen-capturing cells in lymphoid organs from immune mice. Unimmunized, OVA/CFA-immunized, and PBS/CFA-immunized mice were injected with OVA-FITC or PBS into the footpad, and 6 hours later blood, spleen, and LNs were processed for flow cytometry. (A) The histograms show the percentage of OVA-FITC⁺ cells in blood and spleen from OVA/CFA-immunized mice injected with OVA-FITC (black line) or PBS (gray area). (B) Top panels show forward and side light scatter profiles of LN cells. The bottom panel shows the percentage of OVA-FITC⁺ LN cells in OVA-FITC–injected (black line) or in PBS-injected (gray area) mice from the total cell population. A graph showing side light scatter profile of OVA-FITC⁺ cells is also included. Data shown in parentheses depict MFI. One typical experiment of 4 performed is shown (n = 3-4 mice per group).