EDI MoDCs fail to up-regulate surface markers and secrete IL-12 in response to CD40L stimulation. (A) MoDCs from 1 healthy control and 1 patient with EDI (EDI-1) were incubated with medium (gray histogram), CD40L (2.5 μg/mL; blue histogram), CD40L plus IFN-γ (2000 U/mL; red histogram) or LPS (2.5 μg/mL; green histogram) for 24 hours. Cells were immunostained with specific antibodies for surface markers and 10 000 events were collected. Each diagram represents the fluorescence intensity as measured by using a flow cytometer. One representative experiment of 3 is shown. (B) MoDCs from 3 healthy controls and 2 EDI patients were incubated 24 hours with CD40L (2.5 μg/mL) or CD40L plus IFN-γ (2000 U/mL). IL-12p70 secretion was measured in collected supernatants by Luminex (Austin, TX). Data are shown as means ± SEM.