EDI MoDCs fail to form tight and dense clusters when stimulated with CD40L. After 24 hours of stimulation with CD40L (2.5 μg/mL), CD40L plus IFN-γ (2000 U/mL), or LPS (2.5 μg/mL), healthy control (left panel) and EDI patient (right panel) MoDCs were subjected to differential interference contrast (DIC) microscopy, and images were taken at 40 × magnification. One experiment of 2 is shown.