Pretreatment with ACs inhibits NF-κB and IKK activation in NOD but not NOD.MerTK^KD DCs. NOD and NOD.MerTK^KD BMDCs (A-C) or sDCs (D-F) were pretreated with ACs and stimulated with LPS as in Figure 1. (A,D) DNA binding activity of nuclear NF-κB or OCT-1 was determined via EMSA. (B,E) Cytoplasmic IκBα, IκBβ, IκBϵ, and β-actin protein were detected via Western blot using the same blot. (C) In vitro IKK activity and IKK1 and IKK2 protein expression were determined as in Figure 2. (F) IKK phosphorylation was detected via Western blot and the same blot reprobed for IKK1 and IKK2 protein. Data are representative of 3 independent experiments.