αMerTK Ab blocks AC-mediated inhibition of NF-κB activation in NOD and BALB/c DCs. NOD (A-B) or BALB/c (C-D) BMDCs were pretreated with 20 μg/mL of αMerTK or isotype control Ab. BMDCs were then treated with ACs and stimulated with LPS as in Figure 1. (A,C) EMSA was used to measure DNA binding activity of nuclear NF-κB or OCT-1. (B,D) Cytoplasmic IκBα, IκBβ, IκBϵ, and β-actin were detected via Western blot with the same blot. Data are representative of 3 independent experiments.