FOXO1a localization after treatment with solenopsin. 786-O PTEN^−/− cells were infected with AdFKHR and treated with DMSO, wortmannin, or solenopsin. Treatment with the negative control, DMSO, resulted in FOXO1a in the cytoplasm, while treatment with the PI3K inhibitor wortmannin relocalized FOXO1a to the nucleus. Similarly, solenopsin also relocalized FOXO1a to the nucleus. Wortmannin is used as a positive control.