Phospho-Akt and phospho-FOXO1a immunoblot. AdFKHR-infected 786-O cells were treated with DMSO, wortmannin, or solenopsin in decreasing concentrations. Solenopsin, like wortmannin, reduces phospho-Ser473 Akt, phospho-Thr308 Akt, and phospho-Ser256 FOXO1a levels.