Figure 1
Figure 1. Ultrastructure of hagfish endothelium. (A) Electron microscopy (EM) of aorta reveals the presence of many tubular structures within an aortic endothelial cell (EC). (B) EM of aorta shows Weibel-Palade body in EC from aorta. EVS indicates extravascular space. Micrographs in panels A and B were acquired with a 60 ×/1.40 NA oil objective lens (Nikon, Melville, NY), an RT slider SPOT digital camera (Diagnostic Instruments, Sterling Heights, MI), and SPOT version 4.0.9 (Diagnostic Instruments). (C) EM of heart shows electron-lucent endothelium overlying a thick extracellular matrix (ECM) containing a chromaffin-like cell, and a cardiomyocyte with electron-lucent cytoplasm, well-preserved mitochondria, and muscle fibrils. (D) Higher magnification of panel C shows an EC with electron-lucent cytoplasm, a mitochondrion next to a vacuole, and caveola with diaphragm (arrow). (E) EM of heart shows gap in endothelium (double-headed arrow). In panels A to E, blood vessel lumen is on top. (F) EM of dermis reveals a microvessel in cross-section containing 2 nucleated red blood cells (RBCs) with multiple vesicles in the periphery of the cell. EC with nucleus is indicated with an arrow. A cell containing melanin granules is seen in the EVS on the left-hand side of the microvessel. (G) EM of skin shows a Weibel-Palade body (arrow) in a dermal microvascular EC. (H) Higher dermal microvascular endothelium shows well-developed lateral borders of 2 adjacent ECs (EC1 and EC2; arrows). (I) EM of liver sinusoid reveals a large gap in a single endothelial cell (double-headed arrow), well preserved junctions between 2 endothelial cells, thick ECM, and a continuum of proteinaceous material from lumen to extravascular space. EC1 indicates 1 endothelial cell (divided by gap); EC2 is a second endothelial cell. Arrowhead indicates tight and adherens junctional area in the lateral borders of 2 apposed endothelial cells (EC1 and EC2). Scale bar in panel H applies to all panels and represents 865 nm (A), 469 nm (B), 898 nm (C), 258 nm (D), 393 nm (E), 5172 nm (F), 261 nm (G), 2305 nm (H), and 492 nm (I).

Ultrastructure of hagfish endothelium. (A) Electron microscopy (EM) of aorta reveals the presence of many tubular structures within an aortic endothelial cell (EC). (B) EM of aorta shows Weibel-Palade body in EC from aorta. EVS indicates extravascular space. Micrographs in panels A and B were acquired with a 60 ×/1.40 NA oil objective lens (Nikon, Melville, NY), an RT slider SPOT digital camera (Diagnostic Instruments, Sterling Heights, MI), and SPOT version 4.0.9 (Diagnostic Instruments). (C) EM of heart shows electron-lucent endothelium overlying a thick extracellular matrix (ECM) containing a chromaffin-like cell, and a cardiomyocyte with electron-lucent cytoplasm, well-preserved mitochondria, and muscle fibrils. (D) Higher magnification of panel C shows an EC with electron-lucent cytoplasm, a mitochondrion next to a vacuole, and caveola with diaphragm (arrow). (E) EM of heart shows gap in endothelium (double-headed arrow). In panels A to E, blood vessel lumen is on top. (F) EM of dermis reveals a microvessel in cross-section containing 2 nucleated red blood cells (RBCs) with multiple vesicles in the periphery of the cell. EC with nucleus is indicated with an arrow. A cell containing melanin granules is seen in the EVS on the left-hand side of the microvessel. (G) EM of skin shows a Weibel-Palade body (arrow) in a dermal microvascular EC. (H) Higher dermal microvascular endothelium shows well-developed lateral borders of 2 adjacent ECs (EC1 and EC2; arrows). (I) EM of liver sinusoid reveals a large gap in a single endothelial cell (double-headed arrow), well preserved junctions between 2 endothelial cells, thick ECM, and a continuum of proteinaceous material from lumen to extravascular space. EC1 indicates 1 endothelial cell (divided by gap); EC2 is a second endothelial cell. Arrowhead indicates tight and adherens junctional area in the lateral borders of 2 apposed endothelial cells (EC1 and EC2). Scale bar in panel H applies to all panels and represents 865 nm (A), 469 nm (B), 898 nm (C), 258 nm (D), 393 nm (E), 5172 nm (F), 261 nm (G), 2305 nm (H), and 492 nm (I).

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