American Society of Hematology

Figure 1

$Figure 1. Expression of a putative Ly49E ligand by murine tissues and cell lines. (A) Schematic illustration of the pMXs-H/E-IRES-D12 retroviral construct, used to generate BWZ.36 H/ED12 reporter cells expressing the hybrid Ly49H/E together with DAP12. (B) X-gal staining of H/ED12 cells either unstimulated or stimulated as indicated (original magnification ×100). (C,D) CPRG analysis of H/ED12 cells, cocultured with the indicated cell suspensions from murine tissues (C) and with the indicated cell lines (D). Representative data from 1 of 2 to 4 experiments are shown. Bars show CPRG data expressed as a percentage of maximal β-gal induction by phorbol myristate acetate + ionomycin. Background CPRG percentages of the indicated cells in the absence of H/ED12 cells are indicated by the white fraction of each bar.$

Expression of a putative Ly49E ligand by murine tissues and cell lines. (A) Schematic illustration of the pMXs-H/E-IRES-D12 retroviral construct, used to generate BWZ.36 H/ED12 reporter cells expressing the hybrid Ly49H/E together with DAP12. (B) X-gal staining of H/ED12 cells either unstimulated or stimulated as indicated (original magnification ×100). (C,D) CPRG analysis of H/ED12 cells, cocultured with the indicated cell suspensions from murine tissues (C) and with the indicated cell lines (D). Representative data from 1 of 2 to 4 experiments are shown. Bars show CPRG data expressed as a percentage of maximal β-gal induction by phorbol myristate acetate + ionomycin. Background CPRG percentages of the indicated cells in the absence of H/ED12 cells are indicated by the white fraction of each bar.

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