Specific and Ly49E-dependent activation of H/ED12 cells by coated uPA. (A) CPRG analysis of H/ED12 cells incubated in the presence of indicated amounts of uPA. uPA was coated onto the wells (■) or added soluble during incubation (). No (, ■) or anti-Ly49E (4D12) F(ab′)₂ fragments (□) were added. (B) CPRG analysis of the indicated reporter cells incubated in uPA-coated wells (200 ng/well). (C) X-gal staining of H/ED12 cells incubated with uPA KO or WT FD14 intestinal cells. Representative data from 1 of 3 experiments are shown (original magnification ×100). (D) CPRG analysis of H/ED12 cells incubated with the indicated proteins (200 ng/well) or with medium preincubated in uPA-coated wells (200 ng/well) (referred to as uPA-conditioned medium). CPRG data are expressed as mean plus or minus SD of 3 experiments.