Mouse CD47 expression attenuates in vivo phagocytosis of porcine cells. (A) LCL-mCD47 and LCL-neo cells were labeled with CFSE and injected intravenously (1 × 107/mouse) into C57BL/6 mice. At 3 hours after cell injection, spleens were harvested and stained with PE-conjugated anti–mouse F4/80 mAb. Engulfment was seen as a yellow event after merging the green-filtered and red-filtered images (right column). (B) Mice were injected intravenously with a 1:1 mixture of LCL-mCD47 and LCL-neo cells that were labeled with different colors (total 1 × 107 cells per mouse). Spleens were harvested at 3 and 6 hours after cell injection. Pictures shown are representative images taken from an experiment, in which LCL-neo and LCL-mCD47 cells were labeled with PKH-26 (red) and CFSE (green), respectively. Data presented in Figure 5 were representative of 3 or more experiments.
